| 质粒名称: | pSpCas9 (PX165) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 48137 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | PX165 |
| 完整质粒大小: | 8300 bp |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | hSpCas9,SpCas9,Cas9,PX165 |
| 物种来源: | Synthetic |
| 插入大小: | 5400 bp |
| 启动子: | Cbh |
| 标签/融合蛋白: | 3XFLAG（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AgeI(未破坏) |
| 3’克隆位点: | EcoRI (未破坏) |
| 5’测序引物 | CTGGAGCACCTGCCTGAAATCACT |
|
文献引用方法 |
|
| 材料和方法部分: | pSpCas9 (PX165) was a gift from Feng Zhang (Addgene plasmid # 48137 ; http://n2t.net/addgene:48137 ; RRID:Addgene_48137) |
| 参考文献部分: | Genome engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. Epub 2013 Oct 24. 10.1038/nprot.2013.143 PubMed 24157548 |
买家导航
pSpCas9 (PX165)载体质粒基本信息
pSpCas9 (PX165)质粒图谱载体图谱和pSpCas9 (PX165)载体序列质粒序列多克隆位点信息
质粒图谱.png)
pSpCas9 (PX165)质粒载体简介
pSpCas9 (PX165)质粒序列载体序列
LOCUS pSpCas9 (PX165) 8214 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Human codon optimized Cas9 nuclease from Streptococcus pyogenes
(Cbh-3X-FLAG-NLS-SpCas9-NLS).
ACCESSION .
VERSION .
KEYWORDS pSpCas9 (PX165)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8214)
AUTHORS Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
TITLE Genome engineering using the CRISPR-Cas9 system.
JOURNAL Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143.
Epub 2013 Oct 24.
PUBMED 24157548
REFERENCE 2 (bases 1 to 8214)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8214
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 100..119
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
rep_origin complement(136..591)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(273..294)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 485..504
/label=F1ori-R
/note="F1 origin, reverse primer"
repeat_region 666..806
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 677..806
/label=AAV2 ITR
polyA_signal 815..1022
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 1011..1028
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
CDS complement(1056..1103)
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS complement(1104..5204)
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS complement(5229..5249)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(5256..5321)
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
regulatory 5321..5330
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
intron 5342..5569
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
promoter complement(5570..5847)
/label=chicken beta-actin promoter
enhancer 5849..6134
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
repeat_region 6153..6282
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
rep_origin complement(6344..6932)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6424..6443)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(7103..7963)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7726..7745
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7964..8068)
/gene="bla"
/label=AmpR promoter
primer_bind 8136..8154
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(8192..8214)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
61 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
121 tgagagtgca ccataaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
181 gttaaatcag ctcatttttt aaccaataga ccgaaatcgg caaaatccct tataaatcaa
241 aagaatagcc cgagatagag ttgagtgttg ttccagtttg gaacaagagt ccactattaa
301 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
361 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
421 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
481 aggaagggaa gaaagcgaaa ggagcgggcg ctaaggcgct ggcaagtgta gcggtcacgc
541 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tactatggtt
601 gctttgacgt atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
661 gcgcccctgc aggcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg
721 gcgtcgggcg acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt
781 ggccaactcc atcactaggg gttcctgcgg ccgctcccca gcatgcctgc tattctcttc
841 ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact
901 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
961 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
1021 agtcgaggct gatcagcgag ctctaggaat tcttactttt tcttttttgc ctggccggcc
1081 tttttcgtgg ccgccggcct tttgtcgcct cccagctgag acaggtcgat ccgtgtctcg
1141 tacaggccgg tgatgctctg gtggatcagg gtggcgtcca gcacctcttt ggtgctggtg
1201 tacctcttcc ggtcgatggt ggtgtcaaag tacttgaagg cggcaggggc tcccagattg
1261 gtcagggtaa acaggtggat gatattctcg gcctgctctc tgatgggctt atcccggtgc
1321 ttgttgtagg cggacagcac tttgtccaga ttagcgtcgg ccaggatcac tctcttggag
1381 aactcgctga tctgctcgat gatctcgtcc aggtagtgct tgtgctgttc cacaaacagc
1441 tgtttctgct cattatcctc gggggagccc ttcagcttct catagtggct ggccaggtac
1501 aggaagttca catatttgga gggcagggcc agttcgtttc ccttctgcag ttcgccggca
1561 gaggccagca ttctcttccg gccgttttcc agctcgaaca gggagtactt aggcagcttg
1621 atgatcaggt cctttttcac ttctttgtag cccttggctt ccagaaagtc gatgggattc
1681 ttctcgaagc tgcttctttc catgatggtg atccccagca gctctttcac actcttcagt
1741 ttcttggact tgcccttttc cactttggcc accaccagca cagaataggc cacggtgggg
1801 ctgtcgaagc cgccgtactt cttagggtcc cagtccttct ttctggcgat cagcttatcg
1861 ctgttcctct tgggcaggat agactctttg ctgaagccgc ctgtctgcac ctcggtcttt
1921 ttcacgatat tcacttgggg catgctcagc actttccgca cggtggcaaa atcccggccc
1981 ttatcccaca cgatctcccc ggtttcgccg tttgtctcga tcagaggccg cttccggatc
2041 tcgccgttgg ccagggtaat ctcggtcttg aaaaagttca tgatgttgct gtagaagaag
2101 tacttggcgg tagccttgcc gatttcctgc tcgctcttgg cgatcatctt ccgcacgtcg
2161 tacaccttgt agtcgccgta cacgaactcg ctttccagct tagggtactt tttgatcagg
2221 gcggttccca cgacggcgtt caggtaggcg tcgtgggcgt ggtggtagtt gttgatctcg
2281 cgcactttgt aaaactggaa atccttccgg aaatcggaca ccagcttgga cttcagggtg
2341 atcactttca cttcccggat cagcttgtca ttctcgtcgt acttagtgtt catccgggag
2401 tccaggatct gtgccacgtg ctttgtgatc tgccgggttt ccaccagctg tctcttgatg
2461 aagccggcct tatccagttc gctcaggccg cctctctcgg ccttggtcag attgtcgaac
2521 tttctctggg taatcagctt ggcgttcagc agctgccgcc agtagttctt catcttcttc
2581 acgacctctt cggagggcac gttgtcgctc ttgccccggt tcttgtcgct tctggtcagc
2641 accttgttgt cgatggagtc gtccttcaga aagctctgag gcacgatatg gtccacatcg
2701 tagtcggaca gccggttgat gtccagttcc tggtccacgt acatatcccg cccattctgc
2761 aggtagtaca ggtacagctt ctcgttctgc agctgggtgt tttccacggg gtgttctttc
2821 aggatctggc tgcccagctc tttgatgccc tcttcgatcc gcttcattct ctcgcggctg
2881 ttcttctgtc ccttctgggt ggtctggttc tctctggcca tttcgatcac gatgttctcg
2941 ggcttgtgcc ggcccatcac tttcacgagc tcgtccacca ccttcactgt ctgcaggatg
3001 cccttcttaa tggcggggct gccggccaga ttggcaatgt gctcgtgcag gctatcgccc
3061 tggccggaca cctgggcttt ctggatgtcc tctttaaagg tcaggctgtc gtcgtggatc
3121 agctgcatga agtttctgtt ggcgaagccg tcggacttca ggaaatccag gattgtcttg
3181 ccggactgct tgtcccggat gccgttgatc agcttccggc tcagcctgcc ccagccggtg
3241 tatctccgcc gcttcagctg cttcatcact ttgtcgtcga acaggtgggc ataggttttc
3301 agccgttcct cgatcatctc tctgtcctca aacagtgtca gggtcagcac gatatcttcc
3361 agaatgtcct cgttttcctc attgtccagg aagtccttgt ccttgataat tttcagcaga
3421 tcgtggtatg tgcccaggga ggcgttgaac cgatcttcca cgccggagat ttccacggag
3481 tcgaagcact cgattttctt gaagtagtcc tctttcagct gcttcacggt cactttccgg
3541 ttggtcttga acagcaggtc cacgatggcc tttttctgct cgccgctcag gaaggcgggc
3601 tttctcattc cctcggtcac gtatttcact ttggtcagct cgttatacac ggtgaagtac
3661 tcgtacagca ggctgtgctt gggcagcacc ttctcgttgg gcaggttctt atcgaagttg
3721 gtcatccgct cgatgaagct ctgggcggaa gcgcccttgt ccaccacttc ctcgaagttc
3781 cagggggtga tggtttcctc gctctttctg gtcatccagg cgaatctgct gtttcccctg
3841 gccagagggc ccacgtagta ggggatgcgg aaggtcagga tcttctcgat cttttcccgg
3901 ttgtccttca ggaatgggta aaaatcttcc tgccgccgca gaatggcgtg cagctctccc
3961 aggtggatct ggtgggggat gctgccgttg tcgaaggtcc gctgcttccg cagcaggtcc
4021 tctctgttca gcttcacgag cagttcctcg gtgccgtcca tcttttccag gatgggcttg
4081 atgaacttgt agaactcttc ctggctggct ccgccgtcaa tgtagccggc gtagccgttc
4141 ttgctctggt cgaagaaaat ctctttgtac ttctcaggca gctgctgccg cacgagagct
4201 ttcagcaggg tcaggtcctg gtggtgctcg tcgtatctct tgatcataga ggcgctcagg
4261 ggggccttgg tgatctcggt gttcactctc aggatgtcgc tcagcaggat ggcgtcggac
4321 aggttcttgg cggccagaaa caggtcggcg tactggtcgc cgatctgggc cagcaggttg
4381 tccaggtcgt cgtcgtaggt gtccttgctc agctgcagtt tggcatcctc ggccaggtcg
4441 aagttgctct tgaagttggg ggtcaggccc aggctcaggg caatcaggtt tccgaacagg
4501 ccattcttct tctcgccggg cagctgggcg atcagatttt ccagccgtct gctcttgctc
4561 agtctggcag acaggatggc cttggcgtcc acgccgctgg cgttgatggg gttttcctcg
4621 aacagctggt tgtaggtctg caccagctgg atgaacagct tgtccacgtc gctgttgtcg
4681 gggttcaggt cgccctcgat caggaagtgg ccccggaact tgatcatgtg ggccagggcc
4741 agatagatca gccgcaggtc ggccttgtcg gtgctgtcca ccagtttctt tctcaggtgg
4801 tagatggtgg ggtacttctc gtggtaggcc acctcgtcca cgatgttgcc gaagatgggg
4861 tgccgctcgt gcttcttatc ctcttccacc aggaaggact cttccagtct gtggaagaag
4921 ctgtcgtcca ccttggccat ctcgttgctg aagatctctt gcagatagca gatccggttc
4981 ttccgtctgg tgtatcttct tctggcggtt ctcttcagcc gggtggcctc ggctgtttcg
5041 ccgctgtcga acagcagggc tccgatcagg ttcttcttga tgctgtgccg gtcggtgttg
5101 cccagcacct tgaatttctt gctgggcacc ttgtactcgt cggtgatcac ggcccagccc
5161 acagagttgg tgccgatgtc caggccgatg ctgtacttct tgtcggctgc tgggactccg
5221 tggataccga ccttccgctt cttctttggg gccatcttat cgtcatcgtc tttgtaatca
5281 atatcatgat ccttgtagtc tccgtcgtgg tccttatagt ccatggtggc accggtccaa
5341 cctgaaaaaa agtgatttca ggcaggtgct ccaggtaatt aaacattaat accccaccaa
5401 ccaaccatcc cttaaaccct tacctcttgc tcagctaatt acagcccgga ggagaagggc
5461 cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg
5521 aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg
5581 cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga
5641 gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc
5701 cccgccccgc cccgcctggc gcgcgccccc cccccccccc cgcccccatc gctgcacaaa
5761 ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag atggggagag
5821 tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat acgtagatgt
5881 actgccaagt aggaaagtcc cataaggtca tgtactgggc acaatgccag gcgggccatt
5941 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
6001 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
6061 ttactattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
6121 taagttatgt aacgggtacc tctagaggcc gcaggaaccc ctagtgatgg agttggccac
6181 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
6241 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggacatgtga gcaaaaggcc
6301 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
6361 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
6421 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
6481 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
6541 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
6601 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
6661 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
6721 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
6781 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
6841 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
6901 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
6961 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
7021 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
7081 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
7141 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
7201 gggagggctt accatctggc cccagtgctg caatgatacc gcggcttcca cgctcaccgg
7261 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
7321 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
7381 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
7441 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
7501 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
7561 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
7621 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
7681 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
7741 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
7801 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
7861 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
7921 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
7981 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
8041 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
8101 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
8161 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccgg
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pSpCas9 (PX165)质粒载体应用举例
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